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KMID : 0379120120400030152
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Korean Journal of Mycology
2012 Volume.40 No. 3 p.152 ~ p.158
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Production of Lignocellulytic Enzymes from Spent Mushroom Compost of Pleurotus eryngii
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Lim Seon-Hwa
Kim Jong-Kun
Lee Yun-Hae
Kang Hee-Wan
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Abstract
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The lignocellulytic enzymes including a-amylase (EC 3.2.1.1), lignin peroxidase (EC 1.11.1.14), laccase (EC 1.10.3.2), xylanase (EC 3.2.1.8), β-xylosidase (EC 3.2.1.37), β-glucosidase (EC 3.2.1.21) and cellulase (EC 3.2.1.4) were extracted from spent mushroom compost (SMC) of Pleurotus eryngii. Different extraction buffers and conditions were tested for optimal recovery of the enzymes. The optimum extraction was shaking incubation (200 rpm) for 2 h at 4o C. α-Amylase was extracted with the productivity range from 1.20 to 1.6 Unit/SMC g. Cellulase was recovered with the productivity range from 2.10 to 2.80 U/gf. β-glucosidase and β-xylosidase productivities showed lowest recovery producing 0.1 U/g and 0.02 U/g, respectively. The P. eryngii SMCs collected from three different mushroom farms showed different recovery on laccase and xylanse, cellulase. Furthermore, the water extracted SMC was compared to commercial enzymes for its industrial application in decolorization and cellulase activity.
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KEYWORD
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Pleurotus eryngii, Spent mushroom compost, Lignocellulytic enzymes
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